Dr. Viet Loan Dao Thi

VietLoan.DaoThi@med.uni-heidelberg.de

Phone: ++49-(0)6221–56-3546

We study hepatitis E virus-host interactions using stem cell-derived culture systems

Projects

The Dao Thi lab uses stem cell-derived cul­ture mod­els to study hepati­tis E virus (HEV). With about 20 mil­lion infec­tions each year, lead­ing to more than 3 mil­lion symp­to­matic cas­es and ~60’000 fatal­i­ties, HEV is believed to be the most com­mon cause of acute hepati­tis in the world. Despite increas­ing aware­ness, HEV remains an under­stud­ied virus. A con­trib­u­tor to the poor under­stand­ing is the dif­fi­cul­ty to prop­a­gate HEV in cell cul­ture. To over­come these lim­i­ta­tions, we use embry­on­ic or induced pluripo­tent stem cell (hESC/iPSC)-derived cell cul­ture mod­els, in par­tic­u­lar stem-cell derived hepa­to­cyte-like cells. Our stud­ies aim at under­stand­ing dif­fer­ent steps of the HEV life cycle with the over­rid­ing goal to iden­ti­fy new spe­cif­ic tar­gets for ther­a­peu­tic intervention.

For detailed infor­ma­tion on our research, please vis­it our web­site at daothilab.com

Selected Publications

Com­plete pub­li­ca­tion list (PubMed)

Gout­tenoire J, Pol­lán A, Abra­mi L, Oech­slin N, Mau­ron J, Mat­ter M, Oppliger J, Szkol­nic­ka D, Dao Thi VL, van der Goot FG, and Morad­pour D (2018). Palmi­toy­la­tion medi­ates mem­brane asso­ci­a­tion of hepati­tis E virus ORF3 pro­tein and is required for infec­tious par­ti­cle secre­tion. PLoS Pathog.10;14(12):e1007471

 Dao Thi VL, Wu X, and Rice CM (2018). Stem Cell-Derived Cul­ture Mod­els of Hepati­tis E Virus Infec­tion. Cold Spring Harb Per­spect Med. pii: a031799

Chung H, Calis JJA, Wu X, Sun T, Yu Y, Sar­banes SL, Dao Thi VL, Shil­vock AR, Hoff­mann HH, Rosen­berg BR, and Rice CM (2018). Human ADAR1 Pre­vents Endoge­nous RNA from Trig­ger­ing Trans­la­tion­al Shut­down. Cell. 8;172(4):811–824.e14

Wu X*, Dao Thi VL*, Liu P, Takacs CN, Xiang K, Andrus L, Gout­tenoire J, Morad­pour D, and Rice CM (2018). Pan-Geno­type Hepati­tis E Virus Repli­ca­tion in Stem Cell-Derived Hepa­to­cel­lu­lar Sys­tems. Gas­troen­terol­o­gy. 154(3):663–674.e7

Wu X, Dao Thi VL, Huang Y, Biller­beck E, Saha D, Hoff­mann HH, Wang Y, Vale Sil­va LA, Sar­banes S, Sun T, Andrus L, Quirk C, Mac­Don­ald MR, Schnei­der WM, An X, Rosen­berg BR, and Rice CM (2018). Intrin­sic Immu­ni­ty Shapes Viral Resis­tance of Stem Cells. Cell. 172(3):423–438.e25

Dao Thi VL, Debing Y, Wu X, Rice CM, Neyts J, Morad­pour D*, and Gout­tenoire J* (2016) Sofos­bu­vir inhibits Hepati­tis E virus repli­ca­tion in vit­ro and results in an addi­tive effect when com­bined with rib­avirin. Gas­troen­terol­o­gy. 150:82–85