
We study hepatitis E virus-host interactions using stem cell-derived culture systems
Projects
The Dao Thi lab uses stem cell-derived culture models to study hepatitis E virus (HEV). With about 20 million infections each year, leading to more than 3 million symptomatic cases and ~60’000 fatalities, HEV is believed to be the most common cause of acute hepatitis in the world. Despite increasing awareness, HEV remains an understudied virus. A contributor to the poor understanding is the difficulty to propagate HEV in cell culture. To overcome these limitations, we use embryonic or induced pluripotent stem cell (hESC/iPSC)-derived cell culture models, in particular stem-cell derived hepatocyte-like cells. Our studies aim at understanding different steps of the HEV life cycle with the overriding goal to identify new specific targets for therapeutic intervention.
For detailed information on our research, please visit our website at daothilab.com
Selected Publications
Complete publication list (PubMed)
Gouttenoire J, Pollán A, Abrami L, Oechslin N, Mauron J, Matter M, Oppliger J, Szkolnicka D, Dao Thi VL, van der Goot FG, and Moradpour D (2018). Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion. PLoS Pathog.10;14(12):e1007471
Dao Thi VL, Wu X, and Rice CM (2018). Stem Cell-Derived Culture Models of Hepatitis E Virus Infection. Cold Spring Harb Perspect Med. pii: a031799
Chung H, Calis JJA, Wu X, Sun T, Yu Y, Sarbanes SL, Dao Thi VL, Shilvock AR, Hoffmann HH, Rosenberg BR, and Rice CM (2018). Human ADAR1 Prevents Endogenous RNA from Triggering Translational Shutdown. Cell. 8;172(4):811–824.e14
Wu X*, Dao Thi VL*, Liu P, Takacs CN, Xiang K, Andrus L, Gouttenoire J, Moradpour D, and Rice CM (2018). Pan-Genotype Hepatitis E Virus Replication in Stem Cell-Derived Hepatocellular Systems. Gastroenterology. 154(3):663–674.e7
Wu X, Dao Thi VL, Huang Y, Billerbeck E, Saha D, Hoffmann HH, Wang Y, Vale Silva LA, Sarbanes S, Sun T, Andrus L, Quirk C, MacDonald MR, Schneider WM, An X, Rosenberg BR, and Rice CM (2018). Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 172(3):423–438.e25
Dao Thi VL, Debing Y, Wu X, Rice CM, Neyts J, Moradpour D*, and Gouttenoire J* (2016) Sofosbuvir inhibits Hepatitis E virus replication in vitro and results in an additive effect when combined with ribavirin. Gastroenterology. 150:82–85