Host/Pathogen Interactions at Intestinal Epithelial Surfaces
In the lab, we are interested in the intrinsic mechanisms by which human intestinal epithelial cells, on the one hand tolerate the presence of the commensal flora and, on the other hand recognize and respond to pathogen challenges. We use a multidisciplinary approach to investigate fundamental aspects of cellular biology, immunology and virology. We are particularly interested by how the polarized nature of intestinal epithelial cells participates in the maintenance of barrier functions, in the control of viral infection and in the establishment of immune-homeostasis. We exploit epithelium and organoids model systems to recapitulate the complexity of the intestinal tract. We have established model systems to reproduce the cellular and biochemical gut microenvironment allowing us to perform an integrative analysis of host/pathogen interactions at the single cell level.
Our main research questions are:
-How enteric viral pathogens infect the epithelial cells lining the surface of our gastro-intestinal tract.
-How cellular polarity participates in the maintenance of epithelium immune homeostasis.
-How clathrin-mediated endocytosis and trafficking participates in establishment of cellular polarity and polarized immune response.
Hypoxic Environment Promotes Barrier Formation in Human Intestinal Epithelial Cells through Regulation of MicroRNA 320a Expression. Muenchau S, Deutsch R, de Castro IJ, Hielscher T, Heber N, Niesler B, Lusic M, Stanifer ML, Boulant S.Mol Cell Biol. 2019 Jun 27;39(14). pii: e00553-18. doi: 10.1128/MCB.00553–18..
Type-Specific Crosstalk Modulates Interferon Signaling in Intestinal Epithelial Cells. Pervolaraki K, Guo C, Albrecht D, Boulant S, Stanifer ML. J Interferon Cytokine Res. 2019 Jun 13. doi: 10.1089/jir.2019.0040.
Differential induction of interferon stimulated genes between type I and type III interferons is independent of interferon receptor abundance. Pervolaraki K, Rastgou Talemi S, Albrecht D, Bormann F, Bamford C, Mendoza JL, Garcia KC, McLauchlan J, Höfer T, Stanifer ML, Boulant S. PLoS Pathog. 2018 Nov 28;14(11):e1007420. doi: 10.1371/journal.ppat.1007420.
Surface Immobilization of Viruses and Nanoparticles Elucidates Early Events in Clathrin-Mediated Endocytosis. Fratini M, Wiegand T, Funaya C, Jiang Z, Shah PNM, Spatz JP, Cavalcanti-Adam EA, Boulant S.ACS Infect Dis. 2018 Nov 9;4(11):1585–1600. doi: 10.1021/acsinfecdis.8b00134.
Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis. Bucher D, Frey F, Sochacki KA, Kummer S, Bergeest JP, Godinez WJ, Kräusslich HG, Rohr K, Taraska JW, Schwarz US, Boulant S. Nat Commun. 2018 Mar 16;9(1):1109. doi: 10.1038/s41467-018–03533‑0.